alpha 2 Antiplasmin antibody

Catalog No. ABIN458040

This anti-alpha 2 Antiplasmin antibody is a Goat Polyclonal antibody detecting alpha 2 Antiplasmin in ID, IEP and RID. Suitable for Human.

Quick Overview for alpha 2 Antiplasmin antibody (ABIN458040)

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha 2 Antiplasmin antibody is un-conjugated

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Product Details anti-alpha 2 Antiplasmin Antibody

Specificity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal goat antiserum to human alpha-2 antiplasmin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Apha-2 antiplasmin is a single-chain glycoprotein with a molecular weight of about 65,000.The normal concentration in human plasma is 60 µ g/ml. Platelets contain significant amounts of the protein, which is released along with platelet factor 4, following thrombin stimulation. It can form 1:1 complexes with circulating plasmin. Alpha-2 antiplasmin is the most important inhibitor of plasmin-mediated fibrinolysis, but also capable of inactivating factor XIIa, kallikrein, factor XIa, thrombin and factor Xa. The importance of the alpha-2 antiplasmin as regulator of the fibrinolytic system was detected by the discovery of a congenital deficiency. Alpha-2 antiplasmin has been isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony). Detection of plasmin-antiplasmin complexes in plasma is evident of plasmin formation and fibrinolysis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar-block immunodiffusion

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: alpha-2 Antiplasmin

Background: The defined antibody reactivity is restricted to alpha-2 antiplasmin as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones