Kininogen (HMW) antibody (Light Chain)

Catalog No. ABIN458042

The Goat Polyclonal anti-Kininogen (HMW) antibody is suitable to detect Kininogen (HMW) in samples from Human. It has been validated for T and AGE.

Quick Overview for Kininogen (HMW) antibody (Light Chain) (ABIN458042)

Target: Kininogen (HMW) (Kininogen (High Molecular Weight))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Kininogen (HMW) antibody is un-conjugated

Application: Test (T), Agarose Gel Electrophoresis (AGE)

Product Details anti-Kininogen (HMW) Antibody

Binding Specificity: Light Chain

Purpose: Goat anti Human high molecular weight kininogen (light chain specific)

Specificity: Precipitating polyclonal Goat antiSerum to Human high molecular weight kininogen

Cross-Reactivity (Details): The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a reaction with Monkey has been observed.

Characteristics: The defined antibody specificity is to human high molecular weight kininogen. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony, Mancini), using various antiserum concentrations against normal plasma, one single precipitin line is obtained. No precipitation is observed against plasma of patients with high molecular weight kininogen deficiency, but its presence can be demonstrated in normal serum. In double radial immunodiffusion the precipitin line obtained against normal plasma shows a reaction of full identity with the precipitin line with purified high molecular weight kininogen. As precipitating antiserum in electroimmunodiffusion, immunoelectrophoresis and radial immunodiffusion. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Highly purified molecular weight kininogen isolated from pooled plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Precipitation assays. In electroimmunodiffusion (Laurell) the amount of antiserum required in the agarose gel is usually between 1 and 2 % , depending on the test arrangement.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details for Kininogen (HMW)

Target: Kininogen (HMW) (Kininogen (High Molecular Weight))