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Complement Fragment 3a (C3a) antibody Primary Antibody

C3a Reactivity: Human IEP Host: Rabbit Polyclonal
Catalog No. ABIN458140
$444.80
Plus shipping costs $45.00
1 mL
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Delivery in 5 to 7 Business Days
  • Target
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Immunoelectrophoresis (IEP)
    Specificity
    Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.
    Characteristics
    Precipitating polyclonal rabbit antiserum to C3a subunit of human complement C3
    Purification
    Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
    Immunogen
    C3a is a component of complement C3 produced by its activation. It has a molecular weight of 8,900. The protein is isolated and purified from pooled normal human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Target
    Alternative Name
    C3a Subunit of Complement C3 (C3a Antibody Abstract)
    Background
    In immunoelectrophoresis and radial immunodiffusion, using various antiserum concen-trations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma
  • Application Notes
    In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of complement C3a or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:16 when tested against normal human plasma in agar-block immunodiffusi
    Buffer
    Delipidated, heat inactivated, lyophilized, stable whole serum.
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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