APOH antibody

Catalog No. ABIN458152

This Rabbit Polyclonal antibody specifically detects APOH in WB, ELISA, ID and IEP. It exhibits reactivity toward Human.

Quick Overview for APOH antibody (ABIN458152)

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Product Details anti-APOH Antibody

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to human beta-2 glycoprotein 1 (apolipoprotein H)

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Beta-2 glycoprotein 1 has a molecular weight of 40,000. The mean concentration in adult serum is about 200 µ g/ml. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating anti-bodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Measured by quantitative precipitin analysis. The amount of beta2 glycoprotein 1 precipitated by 1 ml a

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for APOH

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Alternative Name: beta-2 Glycoprotein 1 (Apolipoprotein H)

Background: In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma