Lactoferrin antibody (FITC)

Catalog No. ABIN458280

The FITC-conjugated Goat Polyclonal anti-Lactoferrin antibody (ABIN458280) specifically detects Lactoferrin in ELISA, ICC, IHC (fro) and IFA. The antibody is reactive with Monkey samples.

Quick Overview for Lactoferrin antibody (FITC) (ABIN458280)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is conjugated to FITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Product Details anti-Lactoferrin Antibody (FITC)

Purpose: Goat anti Monkey lactoferrin, conjugated with FITC

Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Monkey lactoferrin.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong cross-reaction with lactoferrin in Human milk has been observed.

Characteristics: In immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. As reagent for the direct detection of lactoferrin in monkey cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from monkey milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin

Pathways: Transition Metal Ion Homeostasis