IgA Secretory Component antibody (TRITC)

Catalog No. ABIN458284

This Goat Polyclonal antibody specifically detects IgA Secretory Component in ELISA and IF. It exhibits reactivity toward Monkey.

Quick Overview for IgA Secretory Component antibody (TRITC) (ABIN458284)

Target: IgA Secretory Component

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: TRITC

Application: ELISA, Immunofluorescence (IF)

Product Details

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee.

Characteristics: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to monkey secretory component

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immuno-fluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.5. No foreign proteins added.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)

Background: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein.