Transferrin antibody (Biotin)

Catalog No. ABIN458565

The Biotin-conjugated Rabbit Polyclonal anti-Transferrin antibody (ABIN458565) specifically detects Transferrin in WB, ELISA, IHC (p), ICC and DB. The antibody is reactive with Pig samples.

Quick Overview for Transferrin antibody (Biotin) (ABIN458565)

Target: Transferrin (TF)

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-Transferrin Antibody (Biotin)

Purpose: Rabbit anti Swine transferrin, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Swine transferrin.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin. In enzyme-immunocytochemical and immunohistochemical techniques for the detection of transferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogen: Purified transferrin isolated from pooled swine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin

Pathways: Transition Metal Ion Homeostasis