Albumin antibody

Catalog No. ABIN458582

The Goat Polyclonal anti-Albumin antibody has been validated for ELISA, IEP, ID, Neph and RID. It is suitable to detect Albumin in samples from Rabbit.

Quick Overview for Albumin antibody (ABIN458582)

Target: Albumin (ALB)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Immunodiffusion (ID), Nephelometry (Neph), Radial Immunodiffusion (RID)

Product Details anti-Albumin Antibody

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results:

Characteristics: Precipitating polyclonal goat antiserum to rabbit serum albumin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Highly purified albumin isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As precipitating antiserum to identify or measure rabbit albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal rabbit serum in agar block immunodiffusion titrat

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: The defined antibody specificity is directed to albumin as tested against rabbit sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rabbit serum and the purified albumin.

Pathways: Lipid Metabolism