Albumin antibody

Catalog No. ABIN458707

This Rabbit Polyclonal antibody specifically detects Albumin in ELISA, IEP, ID, Neph and RID. It exhibits reactivity toward Rat and has been mentioned in 4+ publications.

Quick Overview for Albumin antibody (ABIN458707)

Target: Albumin (ALB)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Immunodiffusion (ID), Nephelometry (Neph), Radial Immunodiffusion (RID)

Product Details anti-Albumin Antibody

Specificity: Cow - chicken - dog - goat - guinea pig ++ horse ± human ± mouse + monkey ± rabbit - sheep - swine ±

Characteristics: Precipitating polyclonal rabbit antiserum to rat serum albumin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Highly purified albumin isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As precipitating antiserum to identify or measure rat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal rat serum in agar block immunodiffusion titrati

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

References for anti-Albumin antibody (ABIN458707)

Manogaran, Samardzija, Schad, Wicki, Walker-Egger, Rudin, Grimm, Schippling: "Retinal pathology in experimental optic neuritis is characterized by retrograde degeneration and gliosis." in: Acta neuropathologica communications, Vol. 7, Issue 1, pp. 116, (2019) (PubMed).

Barben, Schori, Samardzija, Grimm: "Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia." in: Molecular neurodegeneration, Vol. 13, Issue 1, pp. 12, (2018) (PubMed).

Geiger, Barben, Grimm, Samardzija: "Blue light-induced retinal lesions, intraretinal vascular leakage and edema formation in the all-cone mouse retina." in: Cell death & disease, Vol. 6, pp. e1985, (2016) (PubMed).

Shen, Choy, Yoshida, Iwase, Hafiz, Xie, Hackett, Arron, Campochiaro: "Interleukin-18 has antipermeablity and antiangiogenic activities in the eye: reciprocal suppression with VEGF." in: Journal of cellular physiology, Vol. 229, Issue 8, pp. 974-83, (2014) (PubMed).

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Serum Albumin

Background: The defined antibody specificity is directed to albumin as tested against rat sera. In immuno-electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin

Pathways: Lipid Metabolism