IgA Secretory Component (Free & Bound) antibody (HRP)

Catalog No. ABIN458765

There is 1 publication for this product available. The Goat Polyclonal anti-IgA Secretory Component antibody is suitable to detect IgA Secretory Component in samples from Monkey. It has been validated for ELISA, WB, IHC (p), ICC and DB.

Quick Overview for IgA Secretory Component (Free & Bound) antibody (HRP) (ABIN458765)

Target: IgA Secretory Component

Reactivity: Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details

Binding Specificity: Free & Bound

Purpose: Goat anti Monkey secretory component (free and bound), conjugated with Horseradish peroxidase

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentRation of the reactants and he sensitivity of the method. This product may also react with free and bound secretory component in many old-world monkeys, including Macaca Sylvanus, Cercopithecus, Cynomolgus, Baboon, and also with the secretory component of Chimpanzees. In double radial immunodiffusion a reaction has been observed with free Human secretory component.

Characteristics: Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal monkey milk, using a high electroendosmosis agar plate, free secretory component ism precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to rhesus Monkey secretory component, free and bound. Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

References

Thomas, Tuero, Demberg, Vargas-Inchaustegui, Musich, Xiao, Venzon, LaBranche, Montefiori, DiPasquale, Reed, DeVico, Fouts, Lewis, Gallo, Robert-Guroff: "HIV-1 CD4-induced (CD4i) gp120 epitope vaccines promote B and T-cell responses that contribute to reduced viral loads in rhesus macaques." in: Virology, Vol. 471-473, pp. 81-92, (2015) (PubMed).

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)