Erythrocytes antibody

Catalog No. ABIN458771

Product Details

Target: Erythrocytes

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Screening Assay (ScA)

Specificity: Inter-species cross-reactivity is a normal feature of antibodies since different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Polyclonal rabbit antiserum to sheep erythrocytes

Purification: Adsorption: No adsorption required. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies since different species frequently share antigenic determinants.

Immunogen: Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: To sensitize cells in hemolytic complement assays and for lymphocytic screening procedures.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Hemolytic titre at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig co

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: Erythrocytes