FITC antibody (Biotin)

Catalog No. ABIN458844

Product Details anti-FITC Antibody (Biotin)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Specificity: This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Characteristics: Biotin-conjugated purified monoclonal mouse antibody to fluorescein isothiocyanate isomer 1

Immunogen: Highly purified fluorescein isothiocyanate isomer 1.

Clone: NI 239

Isotype: IgG1 kappa

Application Details

Application Notes: To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Physicochemical characteristic IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Biotin conjugated purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: Fluorescein Isothiocyanate Isomer 1 (FITC Products)

Target Type: Chemical

Background: The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.