Lactat Dehydrogenase (M4) (LDH-5) antibody

Catalog No. ABIN458930

The Rabbit Polyclonal anti- antibody has been validated for IF, ELISA and WB. It is suitable to detect in samples from Pig.

Quick Overview for Lactat Dehydrogenase (M4) (LDH-5) antibody (ABIN458930)

Target: Lactat Dehydrogenase (M4) (LDH-5)

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Product Details

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Affinity purified antibodies of polyclonal rabbit antiserum to L-lactic dehydrogenase, LDH-5(M 4 ) isoenzyme, from porcine muscle

Purification: Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

Immunogen: L-Lactic dehydrogenase, LDH-5(M 4 ) isoenzyme, isolated and purified from porcine muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: PAb concentration 1 mg/0.5ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: Lactat Dehydrogenase (M4) (LDH-5)

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.