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Phosphodiesterase antibody

Reactivity: Rattlesnake IF, ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN458944
  • Target See all Phosphodiesterase products
    Phosphodiesterase
    Reactivity
    Rattlesnake
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    Affinity purified antibodies of polyclonal rabbit antiserum to phosphodiesterase from Crotalus durissus terrificus venom
    Purification
    Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
    Immunogen
    Phosphodiesterase isolated and purified from Crotalus durissus terrificus venom. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    PAb concentration 1 mg/0.5ml. No foreign proteins added.
    Buffer
    Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Phosphodiesterase
    Abstract
    Phosphodiesterase Products
    Synonyms
    hypothetical protein antibody, Phosphodiesterase antibody, phosphodiesterase antibody, T12B3.3 antibody, pde-6 antibody, lwe1415 antibody
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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