CPK antibody
Quick Overview for CPK antibody (ABIN458973)
Target
See all CPK AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Purpose
- Sheep anti Rabbit Creatine phosphokinase
-
Specificity
- Affinity purified antibodies of polyclonal Sheep antiSerum to creatine phosphokinase from Rabbit muscle.
-
Cross-Reactivity (Details)
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
-
Characteristics
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques),to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
-
Purification
- Affinity purified antibodies
-
Immunogen
- Creatine phosphokinase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
-
-
Application Notes
- Immunoprecipitation.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- It is reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
Buffer
- Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran. No preservative added, as it may interfere with the antibody activity. No foreign protein added.
-
Preservative
- Without preservative
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
-
- CPK (Creatine Phosphokinase (CPK))
-
Alternative Name
- Creatine Phosphokinase
-
UniProt
- P00563
Target
-