CA1 antibody (Biotin)

Catalog No. ABIN459009

Product Details anti-CA1 Antibody (Biotin)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to carbonic anhydrase I from human erythrocytes

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Carbonic anhydrase I isolated and purified from human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 4.9. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is recons tituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: Carbonic Anhydrase I (CA1 Products)

Synonyms: CA-I antibody, CAB antibody, Car1 antibody, AW555628 antibody, Ca1 antibody, Car-1 antibody, BG:DS00941.1 antibody, CAH antibody, CG7820 antibody, Dmel\\CG7820 antibody, Gh7 antibody, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 antibody, ATBCA1 antibody, ATSABP3 antibody, BETA CARBONIC ANHYDRASE 1 antibody, F4P13.5 antibody, F4P13_5 antibody, SABP3 antibody, SALICYLIC ACID-BINDING PROTEIN 3 antibody, carbonic anhydrase 1 antibody, CA1 antibody, LOC100135826 antibody, GB15888 antibody, carbonic anhydrase 1 antibody, Carbonic anhydrase 1 antibody, carbonic anhydrase I antibody, carbonic anhydrase antibody, CA1 antibody, Car1 antibody, CAH1 antibody, LOC100135826 antibody, LOC408827 antibody, Tsp_05114 antibody, LOC100153915 antibody, LOC100050192 antibody, Ca1 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.