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ALT antibody (Biotin)

ALT Reactivity: Pig WB, ELISA, IF Host: Rabbit Polyclonal Biotin
Catalog No. ABIN459041
  • Target See all ALT Antibodies
    ALT (Alanine Aminotransferase (ALT))
    Reactivity
    • 50
    • 23
    • 21
    • 12
    • 6
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Pig
    Host
    • 64
    • 12
    • 7
    • 1
    Rabbit
    Clonality
    • 71
    • 12
    Polyclonal
    Conjugate
    • 47
    • 17
    • 10
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ALT antibody is conjugated to Biotin
    Application
    • 70
    • 36
    • 29
    • 13
    • 11
    • 8
    • 8
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to glutamic-pyruvic transaminase from porcine heart
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Glutamic-pyruvic transaminase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
    Top Product
    Discover our top product ALT Primary Antibody
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 4.2. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    ALT (Alanine Aminotransferase (ALT))
    Alternative Name
    Glutamic-pyruvic Transaminase (ALT Products)
    Synonyms
    AAT1 antibody, ALT1 antibody, GPT1 antibody, GPT antibody, Gpt1 antibody, An11g02620 antibody, AO090003000164 antibody, 233.t00009 antibody, 24.t00016 antibody, 1300007J06Rik antibody, 2310022B03Rik antibody, ALT antibody, Gpt-1 antibody, AU021132 antibody, Dpagt2 antibody, Gnpta antibody, gpt antibody, ALANINE AMINOTRANSFERAS antibody, AtAlaAT1 antibody, AtAlaATc antibody, T13M22.3 antibody, T13M22_3 antibody, alanine aminotransferas antibody, glutamic--pyruvic transaminase antibody, glutamic-pyruvate transaminase (alanine aminotransferase) antibody, alanine aminotransferase antibody, alanine aminotransferase 1 antibody, glutamic pyruvic transaminase, soluble antibody, dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) antibody, alanine aminotransferase 2-like antibody, Alanine aminotransferase 1, mitochondrial antibody, GPT antibody, Gpt antibody, Tb927.1.3950 antibody, ANI_1_368094 antibody, AOR_1_284154 antibody, ALAT_1 antibody, ALAAT1 antibody, EHI_159710 antibody, EHI_096750 antibody, LOC5572541 antibody, ALAT antibody, AAT2 antibody, Dpagt1 antibody, LOC100537633 antibody, AlaAT1 antibody
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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