L-Alanine Dehydrogenase antibody (Biotin)

Catalog No. ABIN459054

Product Details

Target: L-Alanine Dehydrogenase

Reactivity: Bacillus subtilis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-alanine dehydrogenase from Bacillus subtilis

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: L-Alanine dehydrogenase isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.4. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: L-Alanine Dehydrogenase

Synonyms: SCI51.13c antibody, CMS2925 antibody, L-alanine dehydrogenase antibody, l-alanine dehydrogenase antibody, SCO1773 antibody, Mmcs_2104 antibody, Mmar10_1058 antibody, Mmc1_0055 antibody, Acel_1288 antibody, Noca_2488 antibody, ald antibody, Toce_1453 antibody, Ilyop_0407 antibody, Palpr_1204 antibody, Ftrac_3618 antibody, Ocepr_1994 antibody, Sulku_1314 antibody, Tmar_1146 antibody, Intca_1842 antibody, Bache_1992 antibody, Isop_1447 antibody, Despr_2708 antibody, Plabr_0310 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.