LDH5 antibody (Biotin)

Catalog No. ABIN459062

The Rabbit Polyclonal anti-LDH5 antibody has been validated for WB, IF and ELISA. It is suitable to detect LDH5 in samples from Pig.

Quick Overview for LDH5 antibody (Biotin) (ABIN459062)

Target: LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDH5 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-LDH5 Antibody (Biotin)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, from porcine heart

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: L-Lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.4. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for LDH5

Target: LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immuno-diffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.