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LDH5 antibody (Biotin)

LDH5 Reactivity: Rabbit WB, IF, EIA, DB, ID, IP, RIA Host: Sheep Polyclonal Biotin
Catalog No. ABIN568301
  • Target See all LDH5 products
    LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
    Reactivity
    • 4
    • 4
    • 3
    Rabbit
    Host
    • 6
    • 5
    Sheep
    Clonality
    • 11
    Polyclonal
    Conjugate
    • 7
    • 4
    This LDH5 antibody is conjugated to Biotin
    Application
    • 10
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
    Characteristics
    Molar Ratio: Biotin/IgG ~ 6.7
    Purification
    Ammonium Sulphate Precipitation and Ion Exchange Chromatography
    Immunogen
    L-Lactic Dehydrogenase LDH5(M4) isoenzyme, isolated and purified from Rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1.0 mL sterile distilled water
    Concentration
    10 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives and foreign proteins
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target
    LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
    Abstract
    LDH5 Products
    Background
    Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: L-Lactic dehydrogenase LDH-5 Isozyme M4
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