Nuclease P1 antibody (Biotin)

Details for Product No. ABIN459081
Target
Reactivity
Penicillium sp.
7
4
2
Host
Rabbit
11
Clonality
Polyclonal
Conjugate
Biotin
4
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
9
7
5
4
2
2
2
2
2
2
2
Options
Immunogen Nuclease P1 isolated and purified from Penicillium citrinum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificity Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to nuclease P1 from Penicillium citrinum
Purification The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Target
Background The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Application Notes This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
Restrictions For Research Use only
Format Lyophilized
Concentration IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.
Buffer Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to us e, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day . If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.