RNA Polymerase antibody (Biotin)

Catalog No. ABIN459108

The Biotin-conjugated Rabbit Polyclonal anti-RNA Polymerase antibody (ABIN459108) specifically detects RNA Polymerase in WB, ELISA, DB, ICC and IHC (p). The antibody is reactive with E. coli samples.

Quick Overview for RNA Polymerase antibody (Biotin) (ABIN459108)

Target: RNA Polymerase

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNA Polymerase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RNA Polymerase Antibody (Biotin)

Purpose: Rabbit anti Escherichia coli Ribonucleic acid polymerase, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to ribonucleic acid polymerase from Escherichia coli.

Cross-Reactivity (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Purification: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Ribonucleic acid polymerase isolated and purified from Escherichia coli. Freund's complete adjuvant is used in the first step of the immunization procedure

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for RNA Polymerase

Target: RNA Polymerase

Alternative Name: Ribonucleic Acid Polymerase

UniProt: P0A7Z4