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Urate Oxidase (UOX) antibody (Biotin) Primary Antibody

UOX Reactivity: Pig IF, ELISA, WB Host: Rabbit Polyclonal Biotin
Catalog No. ABIN459116
$479.71
Plus shipping costs $45.00
1 mL
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  • Target
    Urate Oxidase (UOX)
    Reactivity
    Pig
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Biotin
    Application
    Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to uricase from porcine liver
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Uricase isolated and purified from porcine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.0. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Urate Oxidase (UOX)
    Alternative Name
    Uricase (UOX Antibody Abstract)
    Synonyms
    AI663847, UOX-2, Uri, Uri2, UOX, urate oxidase, uricase, UOX, Uox, LOC100009266
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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