GAPDH antibody (Biotin)

Catalog No. ABIN459124

This Sheep Polyclonal antibody specifically detects GAPDH in WB, ELISA, ICC, IHC (p) and DB. It exhibits reactivity toward Rabbit.

Quick Overview for GAPDH antibody (Biotin) (ABIN459124)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This GAPDH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Product Details anti-GAPDH Antibody (Biotin)

Purpose: Sheep anti Rabbit Glyceraldehyde-3-phosphate dehydrogenase, conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Sheep antiSerum to glyceraldehyde-3- phosphate dehydrogenase from Rabbit muscle

Cross-Reactivity (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Purification: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: Glyceraldehyde-3-phosphate Dehydrogenase

UniProt: P46406