GDA antibody (Biotin)

Catalog No. ABIN459126

Product Details anti-GDA Antibody (Biotin)

Target: GDA (Guanine Deaminase (GDA))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This GDA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to guanase from rabbit liver

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Guanase isolated and purified from rabbit liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.6. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for GDA

Target: GDA (Guanine Deaminase (GDA))

Alternative Name: Guanase (GDA Products)

Synonyms: CYPIN antibody, GUANASE antibody, NEDASIN antibody, AU015411 antibody, AW047581 antibody, Cypin antibody, MGC81835 antibody, zgc:112282 antibody, AGR_C_4212 antibody, DDBDRAFT_0169373 antibody, DDBDRAFT_0230211 antibody, DDB_0169373 antibody, DDB_0230211 antibody, guanine deaminase antibody, guanine deaminase L homeolog antibody, short-chain dehydrogenase/reductase sdr antibody, GDA antibody, Gda antibody, gda.L antibody, gda antibody, SPO2956 antibody, Gdia_3192 antibody, Mchl_2046 antibody, Mnod_4655 antibody, guaD antibody, Dd586_1771 antibody, Kvar_2512 antibody, Mrub_1462 antibody, BC1002_2263 antibody, Cseg_1079 antibody, Mesil_1795 antibody, Trad_2850 antibody, Mesop_0346 antibody, gDA antibody, ML5_2802 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.