3-Phosphoglyceric Phosphokinase antibody
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- Target
- 3-Phosphoglyceric Phosphokinase
- Reactivity
- Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- IgG fraction of polyclonal rabbit antiserum to 3-phosphoglyceric phosphokinase from baker’s yeast
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- 3-Phosphoglyceric phosphokinase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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Thiol Peroxidases as Major Regulators of Intracellular Levels of Peroxynitrite in Live Saccharomyces cerevisiae Cells." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 5, (2020) (PubMed).
: "Mitochondrial Fusion Machinery Specifically Involved in Energy Deprivation-Induced Autophagy." in: Frontiers in cell and developmental biology, Vol. 8, pp. 221, (2020) (PubMed).
: "Analyses of the three 1-Cys Peroxiredoxins from Aspergillus fumigatus reveal that cytosolic Prx1 is central to H2O2 metabolism and virulence." in: Scientific reports, Vol. 8, Issue 1, pp. 12314, (2019) (PubMed).
: "The yeast arrestin-related protein Bul1 is a novel actor of glucose-induced endocytosis." in: Molecular biology of the cell, Vol. 29, Issue 9, pp. 1012-1020, (2019) (PubMed).
: "The Aspergillus nidulans Pyruvate Dehydrogenase Kinases Are Essential To Integrate Carbon Source Metabolism." in: G3 (Bethesda, Md.), Vol. 8, Issue 7, pp. 2445-2463, (2018) (PubMed).
: "Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES." in: Nature communications, Vol. 9, Issue 1, pp. 1930, (2018) (PubMed).
: "Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis." in: The Journal of cell biology, Vol. 216, Issue 6, pp. 1811-1831, (2017) (PubMed).
: "Gex1 is a yeast glutathione exchanger that interferes with pH and redox homeostasis." in: Molecular biology of the cell, Vol. 22, Issue 12, pp. 2054-67, (2011) (PubMed).
: "
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Thiol Peroxidases as Major Regulators of Intracellular Levels of Peroxynitrite in Live Saccharomyces cerevisiae Cells." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 5, (2020) (PubMed).
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- Target
- 3-Phosphoglyceric Phosphokinase
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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