Polynucleotide Phosphorylase antibody

Catalog No. ABIN459265

Product Details

Target: Polynucleotide Phosphorylase

Reactivity: Micrococcus luteus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to polynucleotide phosphorylase from Micrococcus luteus

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Polynucleotide phosphorylase isolated and purified from Micrococcus luteus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: Polynucleotide Phosphorylase

Synonyms: F20H23.26 antibody, PNP antibody, POLYNUCLEOTIDE PHOSPHORYLASE antibody, resistant to inhibition with FSM 10 antibody, polyribonucleotide nucleotidyltransferase antibody, polynucleotide phosphorylase antibody, RIF10 antibody, Ccan_20500 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.