Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Phosphorylase B antibody

Reactivity: Rabbit ELISA, WB, IF Host: Sheep Polyclonal unconjugated
Catalog No. ABIN459299
  • Target See all Phosphorylase B products
    Phosphorylase B
    Reactivity
    Rabbit
    Host
    • 1
    Sheep
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This Phosphorylase B antibody is un-conjugated
    Application
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. No foreign proteins added.
    Buffer
    Purified hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    Phosphorylase B
    Abstract
    Phosphorylase B Products
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
You are here:
Support