ACE2 antibody (Internal Region)

Catalog No. ABIN461888

The Rabbit Polyclonal anti-ACE2 antibody has been validated for WB, IHC, ELISA and IHC (p). It is suitable to detect ACE2 in samples from Human.

Quick Overview for ACE2 antibody (Internal Region) (ABIN461888)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACE2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACE2 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Anti-ACE2 has no cross response to ACE1.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/mL. A 90 kD band can be detected. Anti-ACE2 has no cross response to ACE1.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2 / ACE-2

Background: Name/Gene ID: ACE2
Subfamily: Metallopeptidase M2
Family: Protease

Synonyms: ACE2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15

Gene ID: 59272

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour