BAK1 antibody

Catalog No. ABIN461962

This Rabbit Polyclonal antibody specifically detects BAK1 in WB, IHC, IF, IP and IHC (p). It exhibits reactivity toward Human, Hamster, Monkey and Pig.

Quick Overview for BAK1 antibody (ABIN461962)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAK1 Antibody

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the sequence of human Bak, conjugated to KLH1.

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IF, IHC, IHC-P (20 μg/mL), IP, WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.2.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: BAK1 / BAK

Background: Name/Gene ID: BAK1

Synonyms: BAK1, Apoptosis regulator BAK, BAK, BCL2L7, Bcl-2-like protein 7, BCL2-antagonist/killer 1, Bcl2-L-7, BCL2-like 7 protein, CDN1, Pro-apoptotic protein BAK, BAK-LIKE

Gene ID: 578

UniProt: Q16611

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response