PLA2G16 antibody (AA 2-15)

Catalog No. ABIN461980

This Goat Polyclonal antibody specifically detects PLA2G16 in ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Monkey and Rabbit.

Quick Overview for PLA2G16 antibody (AA 2-15) (ABIN461980)

Target: PLA2G16 (HRAS-Like Suppressor 3 (PLA2G16))

Reactivity: Human, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PLA2G16 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLA2G16 Antibody

Binding Specificity: AA 2-15

Brand: IHC-plus™

Specificity: Human PLA2G16. Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rabbit (100%) Mouse (93%) Monkey, Rat, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide RAPIPEPKPGDLIE-C from the N-terminus of human PLA2G16 (NP_009000.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rabbit (100%), Mouse (93%), Monkey, Rat, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PLA2G16

Target: PLA2G16 (HRAS-Like Suppressor 3 (PLA2G16))

Alternative Name: PLA2G16 / HRASLS3

Background: Name/Gene ID: PLA2G16

Synonyms: PLA2G16, AdPLA, Adipose-specific PLA2, Group XVI phospholipase A1/A2, Group XVI phospholipase A2, H-REV107-1, HRAS-like suppressor 3, HRASLS3, HREV107-3, HRAS-like suppressor 1, HREV107-1, H-rev 107 protein homolog, HREV107, HRSL3, Phospholipase A2, group XVI

Gene ID: 11145

NCBI Accession: NP_009000

UniProt: P53816

Pathways: Inositol Metabolic Process