SPHK2 antibody (Long Isoform, N-Term)

Catalog No. ABIN4620350

This Rabbit Polyclonal antibody specifically detects SPHK2 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for SPHK2 antibody (Long Isoform, N-Term) (ABIN4620350)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SPHK2 Antibody

Binding Specificity: Long Isoform, N-Term

Specificity: This antibody detetcts Sphingosine Kinase 2 (Long Form).

Purification: Ligand affinity chromatography

Immunogen: Synthetic peptide derived from the N-terminal extension of the Sphingosine Kinase 2-L protein

Isotype: IgG

Application Details

Application Notes: Western blot: (1-5 μg/mL). Positive control: Jurkat lysate. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon arrival, aliquot and freeze at -20 °C to -70 °C.

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: Sphingosine Kinase 2 / SPK2

Background: The long form of sphingosine kinase 2 (Sphk2-L) is a species-specific isoform of the Sphk2 enzyme expressed in human but not in mouse. It is an N-terminal extended form of Sphk2, extended by 36 amino acids. It functions as expected, catalyzing the formation of sphingosine 1-phosphate from sphingosine, however it appears to have a decreased ability to inhibit DNA synthesis as compared to Sphk2-S (short form of Sphk2). Under normal cellular conditions, Sphk2-L does not appear to inhibit DNA synthesis, but under serum deprivation, SphK2-L translocates to the nucleus and accumlates. This accumlation may be involved in the cessation of cell proliferation or apoptosis depending on the cell type.Synonyms: SK2, SPHK2

Gene ID: 56848

NCBI Accession: NP_064511

UniProt: Q9NRA0

Pathways: VEGF Signaling