HMOX1 antibody

Catalog No. ABIN462095

The Mouse Monoclonal anti-HMOX1 antibody has been validated for WB, IHC, FACS and IHC (p). It is suitable to detect HMOX1 in samples from Human, Rat, Mouse, Cow and Dog.

Quick Overview for HMOX1 antibody (ABIN462095)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Human, Rat, Mouse, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5F12-B10

Product Details anti-HMOX1 Antibody

Purification: Protein G purified

Immunogen: Synthetic peptide derived from sequence near the amino-terminus of human HO-1 (Hsp32).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (10 μg/mL), WB (1:250)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: HMOX1 / HO-1

Background: Name/Gene ID: HMOX1

Synonyms: HMOX1, BK286B10, HO-1, HSP32, Heat shock protein, 32-kD, Heme oxygenase (decycling) 1, Heme oxygenase 1, HO1

Gene ID: 3162

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome