CRYAB antibody

Catalog No. ABIN462098

The Mouse Monoclonal anti-CRYAB antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CRYAB in samples from Human, Rat, Mouse, Cow and Chicken.

Quick Overview for CRYAB antibody (ABIN462098)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat, Mouse, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1B6-1-3G4

Product Details anti-CRYAB Antibody

Purification: Protein G purified

Immunogen: CRYAB antibody was raised against bovine otB-Crystallin protein.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB / Alpha B Crystallin

Background: Name/Gene ID: CRYAB

Synonyms: CRYAB, Alpha-crystallin B chain, CRYA2, Crystallin, alpha B, CTPP2, Heat shock protein beta-5, HSPB5, Alpha B Crystallin, Alpha(B)-crystallin, Rosenthal fiber component, Heat-shock 20 kD like-protein

Gene ID: 1410

UniProt: P02511