Hsc70 antibody

Catalog No. ABIN462103

This Rat Monoclonal antibody specifically detects Hsc70 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Sheep, Pig, Rabbit, Guinea Pig and Monkey.

Quick Overview for Hsc70 antibody (ABIN462103)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Sheep, Pig, Rabbit, Guinea Pig, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1B5

Product Details anti-Hsc70 Antibody

Purification: Protein G purified

Immunogen: Native hamster Hsc70 (Hsp73) protein.

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Hamster

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide in 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8 / HSC70

Background: Name/Gene ID: HSPA8

Synonyms: HSPA8, Heat shock 70 kDa protein 8, Heat shock 70kd protein 10, Heat shock 70kD protein 8, Heat shock cognate protein 54, HSP71, HSPA10, HSC71, HSP73, LPS-associated protein 1, Heat shock 70  kDa protein 8, NIP71, HSC54, HSC70, LAP1

Gene ID: 3312

UniProt: P11142