RNH1 antibody (AA 7-461)

Catalog No. ABIN462271

This Mouse Monoclonal antibody specifically detects RNH1 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for RNH1 antibody (AA 7-461) (ABIN462271)

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1H23

Product Details anti-RNH1 Antibody

Binding Specificity: AA 7-461

Brand: IHC-plus™

Specificity: Ribonuclease inhibitor 1(RNH1) (7-461aa)

Purification: Protein G purified

Immunogen: Recombinant Ribonuclease inhibitor 1(RNH1) (7-461aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Extracts of mouse liver (20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for RNH1

Target: RNH1 (Ribonuclease/angiogenin Inhibitor 1 (RNH1))

Alternative Name: RNH1

Background: Name/Gene ID: RNH1

Synonyms: RNH1, Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH

Gene ID: 6050

UniProt: P13489