TDP1 antibody (AA 1-298)

Catalog No. ABIN462272

The Mouse Monoclonal anti-TDP1 antibody has been validated for WB, ELISA, IHC and IHC (p). It is suitable to detect TDP1 in samples from Human.

Quick Overview for TDP1 antibody (AA 1-298) (ABIN462272)

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TDP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1F2

Product Details anti-TDP1 Antibody

Binding Specificity: AA 1-298

Specificity: Human TDP1 (1-298aa)

Purification: Protein G purified

Immunogen: Recombinant human TDP1 (1-298aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TDP1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for TDP1

Target: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Alternative Name: TDP1

Background: Name/Gene ID: TDP1

Synonyms: TDP1, Tyr-DNA phosphodiesterase 1, SCAN1

Gene ID: 55775

UniProt: Q9NUW8