BRCC3 antibody (AA 1-316)

Catalog No. ABIN462274

This Mouse Monoclonal antibody specifically detects BRCC3 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for BRCC3 antibody (AA 1-316) (ABIN462274)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT3B1

Product Details anti-BRCC3 Antibody

Binding Specificity: AA 1-316

Specificity: BRCC36 (1-316aa)

Purification: Protein G purified

Immunogen: Recombinant BRCC36 (1-316aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Alternative Name: BRCC3 / BRCC36

Background: Name/Gene ID: BRCC3

Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2

Gene ID: 79184

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus