MCAM antibody (Tyr641)

Catalog No. ABIN462314

Product Details anti-MCAM Antibody

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Binding Specificity: Tyr641

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Immunoaffinity purified

Immunogen: KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y641 of human MCAM.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Expiry Date: 6 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM / CD146 (MCAM Products)

Background: Name/Gene ID: MCAM
Family: Immunoglobulin

Synonyms: MCAM, CD146, CD146 antigen, Gicerin, Melanoma adhesion molecule, MUC18, p1h12

Gene ID: 4162

UniProt: P43121