TNNT3 antibody

Catalog No. ABIN462336

This anti-TNNT3 antibody is a Mouse Monoclonal antibody detecting TNNT3 in WB, ELISA, IHC, IHC (p) and IHC (fro). Suitable for Human, Chicken and Quail.

Quick Overview for TNNT3 antibody (ABIN462336)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human, Chicken, Quail

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: T1-61

Product Details anti-TNNT3 Antibody

Brand: IHC-plus™

Specificity: Recognizes fast muscle Troponin T.

No Cross-Reactivity: Rabbit

Purification: Protein G purified

Immunogen: Troponin T, purified from chicken breast muscle.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3

Background: Name/Gene ID: TNNT3

Synonyms: TNNT3, AMCD2B, Beta-TnTF, DA2B, FTnT, FSSV, TNTF, Troponin-T3, skeletal, fast, Beta TnTF, Troponin T3, skeletal, fast

Gene ID: 7140

UniProt: P45378