HSPD1 antibody

Catalog No. ABIN462365

The Goat Polyclonal anti-HSPD1 antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect HSPD1 in samples from Human, Mouse, Rat, Monkey, Dog, Hamster, Cow, Rabbit, Pig, Sheep, Drosophila melanogaster, Guinea Pig, Xenopus laevis, Bacteria, Fish and Beluga.

Quick Overview for HSPD1 antibody (ABIN462365)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Monkey, Dog, Hamster, Cow, Rabbit, Pig, Sheep, Drosophila melanogaster, Guinea Pig, Xenopus laevis, Bacteria, Fish, Beluga

Host: Goat

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSPD1 Antibody

Specificity: Hsp60

Purification: Protein G purified

Immunogen: Recombinant human Hsp60 protein.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 / HSP60

Background: Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity