PDIA4 antibody (AA 623-638)

Catalog No. ABIN462442

This Rabbit Polyclonal antibody specifically detects PDIA4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDIA4 antibody (AA 623-638) (ABIN462442)

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDIA4 Antibody

Binding Specificity: AA 623-638

Brand: IHC-plus™

Specificity: Detects an ~72 kD protein, corresponding to the apparent molecular mass of ERp72.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (94%) Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%) Pig, Platypus (81%).

Purification: Protein A purified

Immunogen: A 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (94%), Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%), Pig, Platypus (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for PDIA4

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Alternative Name: PDIA4 / ERP72

Background: Name/Gene ID: PDIA4

Synonyms: PDIA4, ER protein 70, ERp-72, ERP70, ERP72, Protein disulfide-isomerase A4, ER protein 72

Gene ID: 9601

UniProt: P13667

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis