COL6 antibody

Catalog No. ABIN462457

This Goat Polyclonal antibody specifically detects COL6 in ELISA, IHC, IHC (p), IHC (fro), ICC and DB. It exhibits reactivity toward Human.

Quick Overview for COL6 antibody (ABIN462457)

Target: COL6 (Collagen, Type VI (COL6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COL6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-COL6 Antibody

Brand: IHC-plus™

Specificity: Reacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10 % cross-reactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e. g., laminin, fibronectin).

Purification: Immunoaffinity purified

Immunogen: Human type VI collagen.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA, ICC (1:10 - 1:20), IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for COL6

Target: COL6 (Collagen, Type VI (COL6))

Alternative Name: Collagen VI