CCL15 antibody

Catalog No. ABIN464833

This Goat Polyclonal antibody specifically detects CCL15 in ELISA, WB and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL15 antibody (ABIN464833)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL15 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-CCL15 Antibody

Specificity: Human MIP-5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MIP-5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (1.75 - 3 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/mL), a concentration of 1.75-3 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: CCL15 / MIP5

Background: Name/Gene ID: CCL15
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15

Gene ID: 6359

UniProt: Q16663