Defensin beta 3 antibody

Catalog No. ABIN464867

The Rabbit Polyclonal anti-Defensin beta 3 antibody is suitable to detect Defensin beta 3 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Defensin beta 3 antibody (ABIN464867)

Target: Defensin beta 3 (DEFB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Defensin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Defensin beta 3 Antibody

Specificity: Human BD-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BD-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hBD-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-3 (LS-C104286) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-3. Western Blot: To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Defensin beta 3

Target: Defensin beta 3 (DEFB3)

Alternative Name: DEFB3 / beta Defensin 3

Pathways: Production of Molecular Mediator of Immune Response