Galectin 3 antibody

Catalog No. ABIN464873

The Rabbit Polyclonal anti-Galectin 3 antibody is suitable to detect Galectin 3 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Galectin 3 antibody (ABIN464873)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Galectin 3 Antibody

Specificity: Human Galectin-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Galectin-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. ELISA: To detect hGalectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Galectin-3 (LS-C104371) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hGalectin-3. Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: MAC-2 / Galectin 3

Target Type: Chemical

Background: Name/Gene ID: LGALS3

Synonyms: LGALS3, 35 kd lectin, 35 kDa lectin, CBP35, GAL3, Galactoside-binding protein, GALIG, IgE-binding protein, Laminin-binding protein, Gal-3, GALBP, MAC-2, Mac-2 antigen, L31, MAC2, CBP 35, Galactose-specific lectin 3, Galectin-3, L-31, Lectin L-29

Gene ID: 3958

UniProt: P17931

Pathways: RTK Signaling