IGFBP5 antibody (Biotin)

Catalog No. ABIN464890

This Rabbit Polyclonal antibody specifically detects IGFBP5 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for IGFBP5 antibody (Biotin) (ABIN464890)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IGFBP5 Antibody (Biotin)

Specificity: Human IGF-BP5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-BP5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hIGF-BP5 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. To detect hIGF-BP5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP5 (LS-B8207) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP5 / IGFBP-5

Background: Name/Gene ID: IGFBP5

Synonyms: IGFBP5, IBP-5, IGFBP-5, IBP5, IGF-binding protein 5

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding