IL-11 antibody

Catalog No. ABIN465009

The Rabbit Polyclonal anti-IL-11 antibody (ABIN465009) specifically detects IL-11 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL-11 antibody (ABIN465009)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-11 Antibody

Specificity: Human IL-11

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-11.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.15 - 0.25 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/mL overnight at 4 °C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-11 (1.50 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-11 (LS-C104365) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-11. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: IL11

Background: Name/Gene ID: IL11
Family: Hormone

Synonyms: IL11, AGIF, Adipogenesis inhibitory factor, IL-11, Interleukin-11, Oprelvekin, Interleukin 11

Gene ID: 3589

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion