LTA antibody (Biotin)

Catalog No. ABIN465016

This anti-LTA antibody is a Rabbit Polyclonal antibody detecting LTA in WB, ELISA and Neut. Suitable for Human.

Quick Overview for LTA antibody (Biotin) (ABIN465016)

Target: LTA (Lymphotoxin-alpha (LTA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LTA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-LTA Antibody (Biotin)

Specificity: Human TNF-beta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TNF-beta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human TNF-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. To detect Human TNF-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-beta (LS-C104710) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-beta. Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-beta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: LTA / TNF Beta

Target Type: Chemical

Background: Name/Gene ID: LTA
Family: TNF

Synonyms: LTA, Lymphotoxin-alpha, LT-alpha, TNFSF1, TNF-beta, TNFB, Tumor necrosis factor beta, LT, TNF Beta

Gene ID: 4049

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process