IGF2 antibody (Biotin)

Catalog No. ABIN465043

The Biotin-conjugated Rabbit Polyclonal anti-IGF2 antibody (ABIN465043) specifically detects IGF2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for IGF2 antibody (Biotin) (ABIN465043)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IGF2 Antibody (Biotin)

Specificity: Human IGF-II

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-II.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hIGF-II by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-II (LS-B7415) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: IGF2

Background: Name/Gene ID: IGF2
Family: Hormone

Synonyms: IGF2, C11orf43, Insulin-like growth factor II, IGF-II, PP9974, Somatomedin-A

Gene ID: 3481

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation